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normal human diploid fibroblast cells  (ATCC)


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    ATCC normal human diploid fibroblast cells
    Normal Human Diploid Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human diploid fibroblast cells/product/ATCC
    Average 99 stars, based on 5628 article reviews
    normal human diploid fibroblast cells - by Bioz Stars, 2026-03
    99/100 stars

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    Increasing cellular zinc levels in senescent cells enhances PML-NB formation. Normal human fibroblasts IMR90 cells expressing a control vector or the activated RAS oncogene, to induce senescence were treated for 24h with either control vehicle, 5 μM PT (pyrithione) or 1 μM TPEN prior to fixation and indirect immunofluorescence with a specific anti-PML antibody (Bethyl # A301-167A) and with DAPI to stain DNA in the nucleus.

    Journal: Nucleic Acids Research

    Article Title: Zinc controls PML nuclear body formation through regulation of a paralog specific auto-inhibition in SUMO1

    doi: 10.1093/nar/gkac620

    Figure Lengend Snippet: Increasing cellular zinc levels in senescent cells enhances PML-NB formation. Normal human fibroblasts IMR90 cells expressing a control vector or the activated RAS oncogene, to induce senescence were treated for 24h with either control vehicle, 5 μM PT (pyrithione) or 1 μM TPEN prior to fixation and indirect immunofluorescence with a specific anti-PML antibody (Bethyl # A301-167A) and with DAPI to stain DNA in the nucleus.

    Article Snippet: Normal human diploid fibroblasts IMR90 cells were purchased from Coriell Institute for Medical Research (Camden, NJ) and Phoenix Ampho packaging cells were a gift from Dr Scott W Lowe (Sloan Kettering Memorial).

    Techniques: Expressing, Control, Plasmid Preparation, Immunofluorescence, Staining

    Increasing cellular zinc levels in senescent cells enhances PML-NB formation. Normal human fibroblasts IMR90 cells expressing a control vector or the activated RAS oncogene, to induce senescence, were treated for 24 h with either control vehicle, 5 μM pyrithione (PT) or 1 μM TPEN prior to fixation and quantitative analysis for the overall number of PML-NBs per cell ( A ) and the number of PML-NBs greater than 1 μm in size ( B ). For each condition, 50 cells from two independent experiments were counted and statistics were performed using the PRISM9 software.

    Journal: Nucleic Acids Research

    Article Title: Zinc controls PML nuclear body formation through regulation of a paralog specific auto-inhibition in SUMO1

    doi: 10.1093/nar/gkac620

    Figure Lengend Snippet: Increasing cellular zinc levels in senescent cells enhances PML-NB formation. Normal human fibroblasts IMR90 cells expressing a control vector or the activated RAS oncogene, to induce senescence, were treated for 24 h with either control vehicle, 5 μM pyrithione (PT) or 1 μM TPEN prior to fixation and quantitative analysis for the overall number of PML-NBs per cell ( A ) and the number of PML-NBs greater than 1 μm in size ( B ). For each condition, 50 cells from two independent experiments were counted and statistics were performed using the PRISM9 software.

    Article Snippet: Normal human diploid fibroblasts IMR90 cells were purchased from Coriell Institute for Medical Research (Camden, NJ) and Phoenix Ampho packaging cells were a gift from Dr Scott W Lowe (Sloan Kettering Memorial).

    Techniques: Expressing, Control, Plasmid Preparation, Software